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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
22/02/2011 |
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Data da última atualização: |
10/03/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMIRO, D. A.; ESCOUTE, J.; PETITOT, A.-S.; NICOLE, M.; MALUF, M. P.; FERNANDEZ, D. |
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Afiliação: |
INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; CIRAD; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; MIRIAN PEREZ MALUF, SAPC; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT. |
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Título: |
Biphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defense responses in resistant and susceptible coffee cultivars. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In.: PLANT PATHOLOGY, v. 58, p. 944-955, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secreted into, the subsidiary and adjacent cells of the stomata. Additionally, the present study provides new insights into the colonization process of the coffee rust fungus. MenosThe objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secrete... Mostrar Tudo |
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Palavras-Chave: |
Pathogenesis-related genes; Quantitative real-time PCR. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Reactive oxygen species. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29268/1/Biphasic-haustorial.pdf
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Marc: |
LEADER 02423naa a2200229 a 4500
001 1880368
005 2011-03-10
008 2009 bl uuuu u00u1 u #d
100 1 $aRAMIRO, D. A.
245 $aBiphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defense responses in resistant and susceptible coffee cultivars.$h[electronic resource]
260 $c2009
520 $aThe objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secreted into, the subsidiary and adjacent cells of the stomata. Additionally, the present study provides new insights into the colonization process of the coffee rust fungus.
650 $aReactive oxygen species
650 $aCoffea Arábica
653 $aPathogenesis-related genes
653 $aQuantitative real-time PCR
700 1 $aESCOUTE, J.
700 1 $aPETITOT, A.-S.
700 1 $aNICOLE, M.
700 1 $aMALUF, M. P.
700 1 $aFERNANDEZ, D.
773 $tIn.: PLANT PATHOLOGY$gv. 58, p. 944-955, 2009.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 6 | |
| 2. |  | NASCIMENTO JÚNIOR, L.; NEUMAIER, N.; CARMELLO, V.; FÁVARO, F. das N.; SILVA, E. A.; BRENZAM FILHO, F.; TOLEDO, C. F.; DELATTRE, N.; SANTOS, E. L.; OLIVEIRA, M. C. N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Disponibilidade hídrica e rendimentos de grãos de cultivares de soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 280-285. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 3. | | NASCIMENTO JÚNIOR, L.; NEUMAIER, N.; FÁVARO, F. das N.; BREZAN FILHO, F.; SANTOS, J. R. L.; CARMELLO, V.; TOLEDO, C. de F.; DELATTRE, N.; OLIVEIRA, M. C. N. de; FARIAS, J. R. B.; NEPOMUCENO, A. L. Fotossíntese e rendimento de grãos da cultivar de soja BRS 184 sob três níveis de radiação solar. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p. 123, trab. 211. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Biblioteca(s): Embrapa Soja. |
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| 4. | | NASCIMENTO JÚNIOR, L.; NEUMAIER, N.; FÁVARO, F. das N.; BREZAN FILHO, F.; SANTOS, J. R. L.; CARMELLO, V.; TOLEDO, C. de F. T.; DELATTRE, N.; OLIVEIRA, M. C. N. de; FARIAS, J. R. B.; NEPOMUCENO, A. L. Fotossíntese e rendimento de grãos da cultivar de soja BRS 184 sob três níveis de radiação solar. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Anais... Londrina: Embrapa Soja, 2009. Seção trabalhos, t. 211. 1 CD-ROM. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | CARMELLO, V.; NEUMAIER, N.; FÁVARO, F. N.; NASCIMENTO JÚNIOR, L.; BRENZAN FILHO, F.; SANTOS, J. R. L.; TOLEDO, C. F.; DELATTRE, N.; OLIVEIRA, M. C. N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Rendimento de cultivares de soja convencional e transgênica, e disponibilidade hídrica no solo. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 290-295. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | FÁVARO, F. N.; NEUMAIER, N.; CARMELLO, V.; NASCIMENTO JÚNIOR, L.; BRENZAN FILHO, F.; SANTOS, J. R. L.; TOLEDO, C. F.; DELATTRE, N.; OLIVEIRA, M. C. N.; NEPOMUCENO, A. L.; FARIAS, J. R. B. Teor relativo de água e rendimento de grãos de cultivares de soja sob níveis de disponibilidade hídrica no solo. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 192-196. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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